SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis.

@article{citeulike:2636769, abstract = {Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden. The expression profile was obtained from serial analysis of gene expression (SAGE) library data produced from 3- and 6-year-old trees. Fourteen-base expressed sequence tags (ESTs) were searched against public Eucalyptus ESTs and annotated with GenBank. Altogether 43,304 tags were generated producing 3066 unigenes with three or more copies each, 445 with a putative identity, 215 with unknown function and 2406 without an EST match. The expression profile of the juvenile cambial region revealed the presence of highly frequent transcripts related to general metabolism and energy metabolism, cellular processes, transport, structural components and information pathways. We made a quantitative analysis of a large number of genes involved in the biosynthesis of cellulose, pectin, hemicellulose and lignin. Our findings provide insight into the expression of functionally related genes involved in juvenile wood formation in young fast-growing E. grandis trees.}, address = {Laborat\'{o}rio Max Feffer de Gen\'{e}tica de Plantas, Departamento de Gen\'{e}tica, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de S\~{a}o Paulo, Piracicaba-SP, Brazil.}, author = {Gallo de Carvalho, M. C. and Caldas, D. G. and Carneiro, R. T. and Moon, D. H. and Salvatierra, G. R. and Franceschini, L. M. and de Andrade, A. and Celedon, P. A. and Oda, S. and Labate, C. A. }, citeulike-article-id = {2636769}, issn = {0829-318X}, journal = {Tree physiology}, keywords = {cambial, euclayptus, grandis, juvenile, region, sage}, month = {June}, number = {6}, pages = {905--919}, posted-at = {2008-04-07 09:06:09}, priority = {2}, title = {SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18381271}, volume = {28}, year = {2008} }

TY - JOUR ID - citeulike:2636769 L3 - citeulike-article-id:2636769 N2 - Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden. The expression profile was obtained from serial analysis of gene expression (SAGE) library data produced from 3- and 6-year-old trees. Fourteen-base expressed sequence tags (ESTs) were searched against public Eucalyptus ESTs and annotated with GenBank. Altogether 43,304 tags were generated producing 3066 unigenes with three or more copies each, 445 with a putative identity, 215 with unknown function and 2406 without an EST match. The expression profile of the juvenile cambial region revealed the presence of highly frequent transcripts related to general metabolism and energy metabolism, cellular processes, transport, structural components and information pathways. We made a quantitative analysis of a large number of genes involved in the biosynthesis of cellulose, pectin, hemicellulose and lignin. Our findings provide insight into the expression of functionally related genes involved in juvenile wood formation in young fast-growing E. grandis trees. IS - 6 JF - Tree physiology SN - 0829-318X AD - Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba-SP, Brazil. EP - 919 TI - SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis. VL - 28 SP - 905 KW - cambial KW - euclayptus KW - grandis KW - juvenile KW - region KW - sage AU - Gallo de Carvalho, MC AU - Caldas, DG AU - Carneiro, RT AU - Moon, DH AU - Salvatierra, GR AU - Franceschini, LM AU - de Andrade, A AU - Celedon, PA AU - Oda, S AU - Labate, CA PY - 2008/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/18381271 ER -