The influence of temperature on the multiple separation of estrogenic steroids using mobile phases modified with beta-cyclodextrin in high-performance liquid chromatography.

@article{citeulike:2231171, abstract = {The effect of temperature on the retention and multiple separation of six estrogenic steroids in reversed-phase liquid chromatography has been studied. Capacity factors (k') of estriol, 17 beta-estradiol, 17 alpha-estradiol, d-equilenin, equilin and estrone were measured using mobile phase modified with different concentrations of beta-cyclodextrin (from 0-16 mM), a fixed solvent composition (acetonitrile-water) and a wide range of column temperatures (from 5 to 80 degrees C). The plots of capacity factors vs. reciprocal of absolute temperature are nonlinear in each case when mobile phase modified with beta-cyclodextrin was used. Particularly strong nonlinearity was observed at lower temperature and at higher beta-cyclodextrin concentration. The complex chromatograms were evaluated using optimization parameters such as capacity factor of the last-eluted peak (k'max), the smallest resolution between adjacent peaks (Rs,min) and relative resolution product (r). The results presented describe precisely the role of temperature in high-performance liquid chromatography systems in which mobile phases modified with cyclodextrin were used. Moreover, the elution order of estrogenic steroids on modified and unmodified mobile phases has been discussed.}, address = {Medical University of Gdansk, Faculty of Pharmacy, Poland.}, author = {Zarzycki, P. K. and Wierzbowska, M. and Lamparczyk, H. }, citeulike-article-id = {2231171}, issn = {0731-7085}, journal = {J Pharm Biomed Anal}, keywords = {estrogens, hplc}, month = {June}, number = {9-10}, pages = {1281--1287}, posted-at = {2008-01-14 17:19:01}, priority = {2}, title = {The influence of temperature on the multiple separation of estrogenic steroids using mobile phases modified with beta-cyclodextrin in high-performance liquid chromatography.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9226554}, volume = {15}, year = {1997} }

TY - JOUR ID - citeulike:2231171 L3 - citeulike-article-id:2231171 TI - The influence of temperature on the multiple separation of estrogenic steroids using mobile phases modified with beta-cyclodextrin in high-performance liquid chromatography. JF - J Pharm Biomed Anal VL - 15 IS - 9-10 SP - 1281 EP - 1287 AD - Medical University of Gdansk, Faculty of Pharmacy, Poland. SN - 0731-7085 N2 - The effect of temperature on the retention and multiple separation of six estrogenic steroids in reversed-phase liquid chromatography has been studied. Capacity factors (k') of estriol, 17 beta-estradiol, 17 alpha-estradiol, d-equilenin, equilin and estrone were measured using mobile phase modified with different concentrations of beta-cyclodextrin (from 0-16 mM), a fixed solvent composition (acetonitrile-water) and a wide range of column temperatures (from 5 to 80 degrees C). The plots of capacity factors vs. reciprocal of absolute temperature are nonlinear in each case when mobile phase modified with beta-cyclodextrin was used. Particularly strong nonlinearity was observed at lower temperature and at higher beta-cyclodextrin concentration. The complex chromatograms were evaluated using optimization parameters such as capacity factor of the last-eluted peak (k'max), the smallest resolution between adjacent peaks (Rs,min) and relative resolution product (r). The results presented describe precisely the role of temperature in high-performance liquid chromatography systems in which mobile phases modified with cyclodextrin were used. Moreover, the elution order of estrogenic steroids on modified and unmodified mobile phases has been discussed. KW - estrogens KW - hplc AU - Zarzycki, PK AU - Wierzbowska, M AU - Lamparczyk, H PY - 1997/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/9226554 ER -