Construction of a high-resolution comparative gene map between swine chromosome region 6q11-->q21 and human chromosome 19 q-arm by RH mapping of 51 genes.

@article{citeulike:1064640, abstract = {A comprehensive and comparative map was constructed for the porcine chromosome (SSC) 6q11-->q21 region, where the gene(s) responsible for the maldevelopment of embryos are localized using swine populations of the National Institute of Animal Industry, Japan (NIAI). Since the chromosomal region corresponds to a region of human chromosome (HSA) 19q13.1-->q13.3 based on bi-directional chromosome painting, primer pairs were designed from porcine cDNA sequences identified, on a sequence comparison basis, as being transcripts from genes orthologous to those in the HSA region. Fifty-one genes were successfully assigned to a swine radiation hybrid (RH) map with LOD scores greater than 6. ERF and PSMD8 genes were assigned to SSC4 and SSC1, respectively. The remaining 49 genes were assigned to SSC6, demonstrating that the synteny between the SSC6 and HSA19 chromosomal regions is essentially conserved, therefore confirming, the results of bi-directional chromosome painting. However, when examined precisely, rearrangements have apparently occurred within the region of conserved synteny. For the ERF and PSMD8 genes assigned to SSCs other than SSC6, additional mapping using somatic cell hybrid (SCH) panels was performed to confirm the results of RH-mapping.}, address = {Genome Research Department, National Institute of Agrobiological Sciences, Ikenodai, Tsukuba, Ibaraki, Japan.}, author = {Bosak, N. and Faraut, T. and Mikawa, S. and Uenishi, H. and Kiuchi, S. and Hiraiwa, H. and Hayashi, T. and Yasue, H. }, citeulike-article-id = {1064640}, doi = {10.1159/000075734}, issn = {1424-859X}, journal = {Cytogenet Genome Res}, keywords = {lgc, map, porc}, number = {1-4}, pages = {109--115}, posted-at = {2007-01-24 08:50:20}, priority = {2}, title = {Construction of a high-resolution comparative gene map between swine chromosome region 6q11-->q21 and human chromosome 19 q-arm by RH mapping of 51 genes.}, url = {http://dx.doi.org/10.1159/000075734}, volume = {102}, year = {2003} }

TY - JOUR ID - citeulike:1064640 L3 - citeulike-article-id:1064640 TI - Construction of a high-resolution comparative gene map between swine chromosome region 6q11-->q21 and human chromosome 19 q-arm by RH mapping of 51 genes. JF - Cytogenet Genome Res VL - 102 IS - 1-4 SP - 109 EP - 115 AD - Genome Research Department, National Institute of Agrobiological Sciences, Ikenodai, Tsukuba, Ibaraki, Japan. SN - 1424-859X N2 - A comprehensive and comparative map was constructed for the porcine chromosome (SSC) 6q11-->q21 region, where the gene(s) responsible for the maldevelopment of embryos are localized using swine populations of the National Institute of Animal Industry, Japan (NIAI). Since the chromosomal region corresponds to a region of human chromosome (HSA) 19q13.1-->q13.3 based on bi-directional chromosome painting, primer pairs were designed from porcine cDNA sequences identified, on a sequence comparison basis, as being transcripts from genes orthologous to those in the HSA region. Fifty-one genes were successfully assigned to a swine radiation hybrid (RH) map with LOD scores greater than 6. ERF and PSMD8 genes were assigned to SSC4 and SSC1, respectively. The remaining 49 genes were assigned to SSC6, demonstrating that the synteny between the SSC6 and HSA19 chromosomal regions is essentially conserved, therefore confirming, the results of bi-directional chromosome painting. However, when examined precisely, rearrangements have apparently occurred within the region of conserved synteny. For the ERF and PSMD8 genes assigned to SSCs other than SSC6, additional mapping using somatic cell hybrid (SCH) panels was performed to confirm the results of RH-mapping. KW - lgc KW - map KW - porc AU - Bosak, N AU - Faraut, T AU - Mikawa, S AU - Uenishi, H AU - Kiuchi, S AU - Hiraiwa, H AU - Hayashi, T AU - Yasue, H PY - 2003/// UR - http://dx.doi.org/10.1159/000075734 ER -