Thu, 21 Aug 2008 02:20:17 BST CiteULike: xicos Carrolla http://www.citeulike.org/user/xico/author/Carrolla CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/xico/article/227121 <i>Science, Vol. 302, No. 5651. (5 December 2003), pp. 1727-1736.</i><br /><br />Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans. A protein interaction map of Drosophila melanogaster. L Giot JS Bader C Brouwer A Chaudhuri B Kuang Y Li YL Hao CE Ooi B Godwin E Vitols G Vijayadamodar P Pochart H Machineni M Welsh Y Kong B Zerhusen R Malcolm Z Varrone A Collis M Minto S Burgess L McDaniel E Stimpson F Spriggs J Williams K Neurath N Ioime M Agee E Voss K Furtak R Renzulli N Aanensen S Carrolla E Bickelhaupt Y Lazovatsky A DaSilva J Zhong CA Stanyon RL Finley KP White M Braverman T Jarvie S Gold M Leach J Knight RA Shimkets MP McKenna J Chant JM Rothberg doi:10.1126/science.1090289 Science, Vol. 302, No. 5651. (5 December 2003), pp. 1727-1736. 2005-06-14T01:08:12-00:00 2003 Science 1095-9203 302 5651 1727 1736 drosophila fly high-throughput interaction map protein-protein