Thu, 21 Aug 2008 07:13:38 BST CiteULike: sobolevnrms Sailor http://www.citeulike.org/user/sobolevnrm/author/Sailor CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/sobolevnrm/article/2649986 <i>J. Am. Chem. Soc., Vol. 129, No. 19. (16 May 2007), pp. 6064-6065.</i><br /><br />Abstract: The emergent electromagnetic properties of nanoparticle self-assemblies are being harnessed to build new medical and biochemical assays with unprecedented sensitivity. While current self-assembly assays have displayed superior sensitivity for single molecular targets, the development of systems with the capacity to process multiple inputs may more effectively decipher complex disease signatures such as cancer. Herein, we present the design and synthesis of nanoparticles that perform Boolean logic operations using two proteolytic inputs associated with unique aspects of tumorigenesis (MMP2 and MMP7). Using dynamic light scatting, fluorescence, and MRI, we show that logical AND and OR functions can control the self-assembly of disperse superparamagnetic nanoparticles and enable remote, NMR detection of nanoparticle computation. In the future, by increasing the complexity of assembly triggers, nanoparticles may be tailored to sense a diversity of disease inputs in vitro and potentially in vivo. Nanoparticle Self-Assembly Gated by Logical Proteolytic Triggers G Vonmaltzahn TJ Harris JH Park DH Min AJ Schmidt MJ Sailor SN Bhatia doi:10.1021/ja070461l J. Am. Chem. Soc., Vol. 129, No. 19. (16 May 2007), pp. 6064-6065. 2008-04-10T17:20:47-00:00 2007 J. Am. Chem. Soc. 129 19 6064 6065 cancer ccne nanotech http://www.citeulike.org/user/sobolevnrm/article/1331899 <i>Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 3. (2007), pp. 932-6.</i><br /><br />Nanoparticle-based diagnostics and therapeutics hold great promise because multiple functions can be built into the particles. One such function is an ability to home to specific sites in the body. We describe here biomimetic particles that not only home to tumors, but also amplify their own homing. The system is based on a peptide that recognizes clotted plasma proteins and selectively homes to tumors, where it binds to vessel walls and tumor stroma. Iron oxide nanoparticles and liposomes coated with this tumor-homing peptide accumulate in tumor vessels, where they induce additional local clotting, thereby producing new binding sites for more particles. The system mimics platelets, which also circulate freely but accumulate at a diseased site and amplify their own accumulation at that site. The self-amplifying homing is a novel function for nanoparticles. The clotting-based amplification greatly enhances tumor imaging, and the addition of a drug carrier function to the particles is envisioned. Biomimetic amplification of nanoparticle homing to tumors D Simberg T Duza JH Park M Essler J Pilch L Zhang AM Derfus M Yang RM Hoffman S Bhatia MJ Sailor E Ruoslahti Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 3. (2007), pp. 932-6. 2007-05-25T13:36:03-00:00 2007 Proceedings of the National Academy of Sciences of the United States of America 104 3 932 6 drugdesign nanotech