Thu, 21 Aug 2008 14:50:33 BST CiteULike: RMGrazes Yvert http://www.citeulike.org/user/RMGraze/author/Yvert CiteULike.org en-gb Copyright © 2004-2008 citeulike.org http://www.citeulike.org/user/RMGraze/article/620656 <i>Science, Vol. 296, No. 5568. (26 April 2002), pp. 752-755.</i><br /><br />10.1126/science.1069516 Genetic Dissection of Transcriptional Regulation in Budding Yeast Rachel Brem Gael Yvert Rebecca Clinton Leonid Kruglyak doi:10.1126/science.1069516 Science, Vol. 296, No. 5568. (26 April 2002), pp. 752-755. 2006-05-09T19:40:57-00:00 2002 Science 296 5568 752 755 cis_trans yeast http://www.citeulike.org/user/RMGraze/article/143676 <i>Genome Res, Vol. 15, No. 2. (February 2005), pp. 284-291.</i><br /><br />Oligonucleotide microarrays provide a high-throughput method for exploring genomes. In addition to their utility for gene-expression analysis, oligonucleotide-expression arrays have also been used to perform genotyping on genomic DNA. Here, we show that in segregants from a cross between two unrelated strains of Saccharomyces cerevisiae, high-quality genotype data can also be obtained when mRNA is hybridized to an oligonucleotide-expression array. We were able to identify and genotype nearly 1000 polymorphisms at an error rate close to 3% in segregants and at an error rate of 7% in diploid strains, a performance comparable to methods using genomic DNA. In addition, we demonstrate how simultaneous genotyping and gene-expression profiling can reveal cis-regulatory variation by screening hundreds of genes for allele-specific expression. With this method, we discovered 70 ORFs with evidence for preferential expression of one allele in a diploid hybrid of two S. cerevisiae strains. Simultaneous genotyping, gene-expression measurement, and detection of allele-specific expression with oligonucleotide arrays. J Ronald JM Akey J Whittle EN Smith G Yvert L Kruglyak doi:10.1101/gr.2850605 Genome Res, Vol. 15, No. 2. (February 2005), pp. 284-291. 2005-03-31T21:18:40-00:00 2005 Genome Res 1088-9051 15 2 284 291 cis_trans http://www.citeulike.org/user/RMGraze/article/620661 <i>Nat Genet, Vol. 35, No. 1. (2003), pp. 57-64.</i> Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors Gael Yvert Rachel Brem Jacqueline Whittle Joshua Akey Eric Foss Erin Smith Rachel Mackelprang Leonid Kruglyak doi:10.1038/ng1222 Nat Genet, Vol. 35, No. 1. (2003), pp. 57-64. 2006-05-09T19:48:36-00:00 2003 Nat Genet 35 1 57 64 cis_trans yeast