Effect of Fructose Overfeeding and Fish Oil Administration on Hepatic De Novo Lipogenesis and Insulin Sensitivity in Healthy Men

@article{citeulike:1020026, abstract = {High-fructose diet stimulates hepatic de novo lipogenesis (DNL) and causes hypertriglyceridemia and insulin resistance in rodents. Fructose-induced insulin resistance may be secondary to alterations of lipid metabolism. In contrast, fish oil supplementation decreases triglycerides and may improve insulin resistance. Therefore, we studied the effect of high-fructose diet and fish oil on DNL and VLDL triglycerides and their impact on insulin resistance. Seven normal men were studied on four occasions: after fish oil (7.2 g/day) for 28 days; a 6-day high-fructose diet (corresponding to an extra 25\% of total calories); fish oil plus high-fructose diet; and control conditions. Following each condition, fasting fractional DNL and endogenous glucose production (EGP) were evaluated using [1-13C]sodium acetate and 6,6-2H2 glucose and a two-step hyperinsulinemic-euglycemic clamp was performed to assess insulin sensitivity. High-fructose diet significantly increased fasting glycemia (7 {+/-} 2\%), triglycerides (79 {+/-} 22\%), fractional DNL (sixfold), and EGP (14 {+/-} 3\%, all P < 0.05). It also impaired insulin-induced suppression of adipose tissue lipolysis and EGP (P < 0.05) but had no effect on whole- body insulin-mediated glucose disposal. Fish oil significantly decreased triglycerides (37\%, P < 0.05) after high-fructose diet compared with high-fructose diet without fish oil and tended to reduce DNL but had no other significant effect. In conclusion, high-fructose diet induced dyslipidemia and hepatic and adipose tissue insulin resistance. Fish oil reversed dyslipidemia but not insulin resistance.}, author = {Faeh, David and Minehira, Kaori and Schwarz, Jean-Marc and Periasamy, Raj and Park, Seongsoo and Tappy, Luc }, citeulike-article-id = {1020026}, journal = {Diabetes}, keywords = {diet, fructose, health, insulin, omega3}, month = {July}, number = {7}, pages = {1907--1913}, posted-at = {2006-12-30 23:39:42}, priority = {5}, title = {Effect of Fructose Overfeeding and Fish Oil Administration on Hepatic De Novo Lipogenesis and Insulin Sensitivity in Healthy Men}, url = {http://diabetes.diabetesjournals.org/cgi/content/abstract/54/7/1907}, volume = {54}, year = {2005} }

TY - JOUR ID - citeulike:1020026 L3 - citeulike-article-id:1020026 N2 - High-fructose diet stimulates hepatic de novo lipogenesis (DNL) and causes hypertriglyceridemia and insulin resistance in rodents. Fructose-induced insulin resistance may be secondary to alterations of lipid metabolism. In contrast, fish oil supplementation decreases triglycerides and may improve insulin resistance. Therefore, we studied the effect of high-fructose diet and fish oil on DNL and VLDL triglycerides and their impact on insulin resistance. Seven normal men were studied on four occasions: after fish oil (7.2 g/day) for 28 days; a 6-day high-fructose diet (corresponding to an extra 25% of total calories); fish oil plus high-fructose diet; and control conditions. Following each condition, fasting fractional DNL and endogenous glucose production (EGP) were evaluated using [1-13C]sodium acetate and 6,6-2H2 glucose and a two-step hyperinsulinemic-euglycemic clamp was performed to assess insulin sensitivity. High-fructose diet significantly increased fasting glycemia (7 {+/-} 2%), triglycerides (79 {+/-} 22%), fractional DNL (sixfold), and EGP (14 {+/-} 3%, all P < 0.05). It also impaired insulin-induced suppression of adipose tissue lipolysis and EGP (P < 0.05) but had no effect on whole- body insulin-mediated glucose disposal. Fish oil significantly decreased triglycerides (37%, P < 0.05) after high-fructose diet compared with high-fructose diet without fish oil and tended to reduce DNL but had no other significant effect. In conclusion, high-fructose diet induced dyslipidemia and hepatic and adipose tissue insulin resistance. Fish oil reversed dyslipidemia but not insulin resistance. IS - 7 JF - Diabetes EP - 1913 TI - Effect of Fructose Overfeeding and Fish Oil Administration on Hepatic De Novo Lipogenesis and Insulin Sensitivity in Healthy Men VL - 54 SP - 1907 KW - diet KW - fructose KW - health KW - insulin KW - omega3 AU - Faeh, David AU - Minehira, Kaori AU - Schwarz, Jean-Marc AU - Periasamy, Raj AU - Park, Seongsoo AU - Tappy, Luc PY - 2005/07/01/ UR - http://diabetes.diabetesjournals.org/cgi/content/abstract/54/7/1907 ER -