A Protein-Protein Interaction Network for Human Inherited Ataxias and Disorders of Purkinje Cell Degeneration

@article{citeulike:657918, abstract = {SummaryMany human inherited neurodegenerative disorders are characterized by loss of balance due to cerebellar Purkinje cell (PC) degeneration. Although the disease-causing mutations have been identified for a number of these disorders, the normal functions of the proteins involved remain, in many cases, unknown. To gain insight into the function of proteins involved in PC degeneration, we developed an interaction network for 54 proteins involved in 23 inherited ataxias and expanded the network by incorporating literature-curated and evolutionarily conserved interactions. We identified 770 mostly novel protein-protein interactions using a stringent yeast two-hybrid screen; of 75 pairs tested, 83\% of the interactions were verified in mammalian cells. Many ataxia-causing proteins share interacting partners, a subset of which have been found to modify neurodegeneration in animal models. This interactome thus provides a tool for understanding pathogenic mechanisms common for this class of neurodegenerative disorders and for identifying candidate genes for inherited ataxias.}, author = {Lim, Janghoo and Hao, Tong and Shaw, Chad and Patel, Akash J. and Szabo, Gabor and Rual, Jean-Francois and Fisk, Joseph C. and Li, Ning and Smolyar, Alex and Hill, David E. }, citeulike-article-id = {657918}, doi = {http://dx.doi.org/10.1016/j.cell.2006.03.032}, journal = {Cell}, keywords = {biology, networks}, month = {May}, number = {4}, pages = {801--814}, posted-at = {2007-11-27 10:31:22}, priority = {4}, title = {A Protein-Protein Interaction Network for Human Inherited Ataxias and Disorders of Purkinje Cell Degeneration}, url = {http://dx.doi.org/10.1016/j.cell.2006.03.032}, volume = {125}, year = {2006} }

TY - JOUR ID - citeulike:657918 L3 - citeulike-article-id:657918 N2 - SummaryMany human inherited neurodegenerative disorders are characterized by loss of balance due to cerebellar Purkinje cell (PC) degeneration. Although the disease-causing mutations have been identified for a number of these disorders, the normal functions of the proteins involved remain, in many cases, unknown. To gain insight into the function of proteins involved in PC degeneration, we developed an interaction network for 54 proteins involved in 23 inherited ataxias and expanded the network by incorporating literature-curated and evolutionarily conserved interactions. We identified 770 mostly novel protein-protein interactions using a stringent yeast two-hybrid screen; of 75 pairs tested, 83% of the interactions were verified in mammalian cells. Many ataxia-causing proteins share interacting partners, a subset of which have been found to modify neurodegeneration in animal models. This interactome thus provides a tool for understanding pathogenic mechanisms common for this class of neurodegenerative disorders and for identifying candidate genes for inherited ataxias. IS - 4 JF - Cell EP - 814 TI - A Protein-Protein Interaction Network for Human Inherited Ataxias and Disorders of Purkinje Cell Degeneration VL - 125 SP - 801 KW - biology KW - networks AU - Lim, Janghoo AU - Hao, Tong AU - Shaw, Chad AU - Patel, Akash AU - Szabo, Gabor AU - Rual, Jean-Francois AU - Fisk, Joseph AU - Li, Ning AU - Smolyar, Alex AU - Hill, David PY - 2006/05/19/ UR - http://dx.doi.org/10.1016/j.cell.2006.03.032 ER -