Genetic reconstruction of a functional transcriptional regulatory network.

@article{citeulike:1219932, abstract = {Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors.}, address = {[1] Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, 1 University Station A4800, Austin, Texas 78712, USA. [2] Current address: Dental Research Institute, University of California at Los Angeles, Los Angeles, California 90095-1668, USA. [3] These authors contributed equally to this work.}, author = {Hu, Zhanzhi and Killion, Patrick J J. and Iyer, Vishwanath R R. }, citeulike-article-id = {1219932}, doi = {10.1038/ng2012}, issn = {1061-4036}, journal = {Nat Genet}, keywords = {expression, tf, yeast}, month = {April}, posted-at = {2007-06-13 09:45:15}, priority = {2}, title = {Genetic reconstruction of a functional transcriptional regulatory network.}, url = {http://dx.doi.org/10.1038/ng2012}, year = {2007} }

TY - JOUR ID - citeulike:1219932 L3 - citeulike-article-id:1219932 TI - Genetic reconstruction of a functional transcriptional regulatory network. JF - Nat Genet AD - [1] Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, 1 University Station A4800, Austin, Texas 78712, USA. [2] Current address: Dental Research Institute, University of California at Los Angeles, Los Angeles, California 90095-1668, USA. [3] These authors contributed equally to this work. SN - 1061-4036 N2 - Although global analyses of transcription factor binding provide one view of potential transcriptional regulatory networks, regulation also occurs at levels distinct from transcription factor binding. Here, we use a genetic approach to identify targets of transcription factors in yeast and reconstruct a functional regulatory network. First, we profiled transcriptional responses in S. cerevisiae strains with individual deletions of 263 transcription factors. Then we used directed-weighted graph modeling and regulatory epistasis analysis to identify indirect regulatory relationships between these transcription factors, and from this we reconstructed a functional transcriptional regulatory network. The enrichment of promoter motifs and Gene Ontology annotations provide insight into the biological functions of the transcription factors. KW - expression KW - tf KW - yeast AU - Hu, Zhanzhi AU - Killion, Patrick J AU - Iyer, Vishwanath R PY - 2007/04/08/ UR - http://dx.doi.org/10.1038/ng2012 ER -